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anti pperk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti pperk
    Anti Pperk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-pperk/pm41596608-269-10-18
    Average 86 stars, based on 1 article reviews
    anti pperk - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: The p53 target DRAM1 modulates calcium homeostasis and ER stress by promoting contact between lysosomes and the ER through STIM1
    Article Snippet: Following blocking with 5% nonfat milk at room temperature for 1 h, membranes were incubated with following primary antibodies at 4°C overnight (Anti-DRAM1, Abclonal A15468, 1:1000; Anti-ACTB, Proteintech, 1:3000; Anti-Bax, Santa Cruz sc-526, 1:300; Anti-P53, Santa Cruz sc-6243, 1:300; Anti-Beclin1, Proteintech 11306-1-AP, 1:1000; Anti-CHOP, Proteintech 15204-1-AP, 1:1000; Anti-GRP78, Proteintech 11587-1-AP, 1:1000; Anti-LC3, Proteintech 14600-1-AP, 1:1000; Anti-P62, Proteintech 18420-1-AP, 1:1000; Anti-ATF4, Proteintech 10835-1-AP, 1:1000; Anti-EIF2α, Proteintech 11233-1-AP, 1:1000; Anti-p-EIF2α, Cell signaling technology 9721, 1:1000; Anti-pPERK, Cell signaling technology 3197, 1:1000; Anti-Calnexin, Proteintech 10427-2-AP, 1:1000; Anti-Calreticulin, Proteintech 27298-1-AP, 1:1000; Anti-KTN1, Proteintech 19841-1-AP, 1:1000; Anti-Ribophorin, Proteintech 12894-1-AP, 1:1000; Anti-C53, Proteintech 11007-1-AP, 1:1000; Anti-ZMPSTE24, Proteintech 12847-1-AP, 1:1000; Anti-FAM134, Proteintech 21537-1-AP, 1:1000; Anti-TEX264, Proteintech 25858-1-AP, 1:1000; Anti-CKAP4, Proteintech 16686-1-AP, 1:1000; Anti-Sec62, Proteintech 28693-1-AP, 1:1000; Anti-GP78, Proteintech 16675-1-AP, 1:1000; Anti-ATG10, Prosci 4399, 1:1000; Anti-ATG16, Prosci 4425, 1:1000; Anti-LAMP2, Prosci 3627, 1:1000; Anti-p-4E-BP1, Cell signaling technology 13443, 1:1000; Anti-AMPK, Cell signaling technology 2532, 1:1000; Anti-p-AMPK, Cell signaling technology 2535, 1:1000; AntiTFEB, Proteintech 13372-1-AP, 1:1000; Anti-mTOR, Proteintech 66888-1-Ig, 1:1000; AntiSTIM1, Proteintech 11565-1-AP, 1:1000; Anti-VAPB, Proteintech 14477-1-AP/66191-1-Ig, 1:1000; Anti-TMEM192, Proteintech 28263-1-AP, 1:1000; Anti-ORAI1, Proteintech 66223-1-Ig, 1:1000; Anti-ULK1, Proteintech 29005-1-AP, 1:1000; Anit-p-ULK1, Proteintech 80218-1-RR, 1:1000).

    Article Title: A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis Pathway
    Article Snippet: The following antibodies and dilutions were used: anti-CHOP/GADD153 1:2000, anti-ATF4 1:2000; anti-XBP-1 1:2000; anti-JNK1 1:5000 (Santa Cruz); anti-caspase-12 1:3000 (Exalpha Biologicals), anti-BAX and anti-BAK 1∶1000 (Upstate Technology); anti-HA 1∶2000 (Roche), anti-caspase-9 1:1000 (Pharmigen), anti-actin 1∶5000 (Chemicon Inc.), anti-SERCA 1∶1000 (Affinity Bioreagents), anti-HSP90 1:1000 (Santa Cruz Biotechnology) and anti-pPERK, and p-JNK 1∶2000 (Cell Signaling).

    Article Title: RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK–CHOP signaling
    Article Snippet: Primary antibodies included anti-RCN1 (Bethyl, Montgomery, TX, USA), anti-GFP (MBL, Nagoya, Japan), anti-Flag (Sigma), anti-IP 3 R1 (Bethyl), anti-tubulin (Sigma), anti-ATF4 (GeneTex, San Antonio, TX, USA), anti-pIRE1 (Abcam, Cambridge, MA, USA), anti-pPERK, anti-caspase-3, anti-PERK, anti-IRE1, anti-GAPDH, anti-CHOP, anti-eIF2α and anti-GRP78/BiP (Cell Signaling Technology, Danvers, MA, USA).

    Article Title: Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice
    Article Snippet: Dilution ratio of primary antibodies used: anti-p-AKT (1:1000; CST, 4058S), anti-AKT (1:1000; CST, 9272S), anti-p-GSK3β (1:1000; Proteintech, 67558-1-Ig), anti-GSK3β (1:1000; Proteintech, 22104-1-AP), anti-p-FOXO1/FOXO3a (1:1000; Beyotime, AF605), anti-FOXO1 (1:1000; Beyotime, AF603), anti-β-actin (1:1000; ZSbio, TA-09), anti-p-IGF-1Rβ/IRβ (1:1000; CST, 3024T), anti-IGF-1Rβ (1:1000; CST, 9750T), anti-p-SRC (1:1000; CST, 6943S), anti-SRC (1:1000; CST, 2109S), anti-p-EGFR (1:1000; CST, 2234S), anti-EGFR (1:1000; CST, 4267S), anti-CAV1 (1:1000; CST, 3267S), anti-CC3 (1:1000; CST, 9661S), anti-CHOP (1:1000; Proteintech, 15204-1-AP), anti-pPERK (1:1000; CST, 3179S), anti-PERK (1:1000; CST, 3192S), anti-β-Tubulin (1:2000; ZSbio, TA-10).

    Article Title: Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling
    Article Snippet: The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and anti-β-arrestin2 antibodies were from Cell Signaling Technology (Boston, MA, USA); anti-E-cadherin, anti-N-cadherin, anti-snai1, and anti-β-actin antibodies were purchased from Santa Cruz (Dallas, TX, USA).

    Article Title: Dual role of sphingosine kinase-1 in promoting the differentiation of dermal fibroblasts and the dissemination of melanoma cells.
    Article Snippet: Despite progress in the understanding of the biology and genetics of melanoma, no effective treatment against this cancer is available.. The adjacent microenvironment has an important role in melanoma progression.. Defining the molecular signals that control the bidirectional dialog between malignant cells and the surrounding stroma is crucial for efficient targeted therapy.

    Article Title: Insulin Secretion and Ca 2+ Dynamics in β-Cells Are Regulated by PERK (EIF2AK3) in Concert with Calcineurin
    Article Snippet: Primary antibodies used in the analysis were: anti-eIF2α-P (1:500, Invitrogen), anti-tubulin (1:1000, Sigma), anti-PERK (1:500, Cell Signaling), anti-pPERK (1:500, Cell Signaling), anti-SERCA N1 (1:5000), and anti-calnexin (1:1000, Enzo Life Sciences).

    Article Title: TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways
    Article Snippet: For anti-BCL-2 (Abcam), anti-BAX (Abcam), and anti-pPERK (rabbit monoclonal antibody; 1:1000, v / v ) (Cell Signaling Technology, Danvers, MA, USA), after overnight incubation, the membranes were washed three times with Tris-buffered saline containing 0.1% ( v / v ) Tween 20 (TBST) for 5 min each, after which they were incubated with secondary antibody [Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488); 1:500 v / v ] for 1 h. After incubation, the membranes were washed three times with TBST buffer and immediately visualized using a ChemiDoc system (Bio-Rad Laboratories).

    Incubation:

    Article Title: The p53 target DRAM1 modulates calcium homeostasis and ER stress by promoting contact between lysosomes and the ER through STIM1
    Article Snippet: Following blocking with 5% nonfat milk at room temperature for 1 h, membranes were incubated with following primary antibodies at 4°C overnight (Anti-DRAM1, Abclonal A15468, 1:1000; Anti-ACTB, Proteintech, 1:3000; Anti-Bax, Santa Cruz sc-526, 1:300; Anti-P53, Santa Cruz sc-6243, 1:300; Anti-Beclin1, Proteintech 11306-1-AP, 1:1000; Anti-CHOP, Proteintech 15204-1-AP, 1:1000; Anti-GRP78, Proteintech 11587-1-AP, 1:1000; Anti-LC3, Proteintech 14600-1-AP, 1:1000; Anti-P62, Proteintech 18420-1-AP, 1:1000; Anti-ATF4, Proteintech 10835-1-AP, 1:1000; Anti-EIF2α, Proteintech 11233-1-AP, 1:1000; Anti-p-EIF2α, Cell signaling technology 9721, 1:1000; Anti-pPERK, Cell signaling technology 3197, 1:1000; Anti-Calnexin, Proteintech 10427-2-AP, 1:1000; Anti-Calreticulin, Proteintech 27298-1-AP, 1:1000; Anti-KTN1, Proteintech 19841-1-AP, 1:1000; Anti-Ribophorin, Proteintech 12894-1-AP, 1:1000; Anti-C53, Proteintech 11007-1-AP, 1:1000; Anti-ZMPSTE24, Proteintech 12847-1-AP, 1:1000; Anti-FAM134, Proteintech 21537-1-AP, 1:1000; Anti-TEX264, Proteintech 25858-1-AP, 1:1000; Anti-CKAP4, Proteintech 16686-1-AP, 1:1000; Anti-Sec62, Proteintech 28693-1-AP, 1:1000; Anti-GP78, Proteintech 16675-1-AP, 1:1000; Anti-ATG10, Prosci 4399, 1:1000; Anti-ATG16, Prosci 4425, 1:1000; Anti-LAMP2, Prosci 3627, 1:1000; Anti-p-4E-BP1, Cell signaling technology 13443, 1:1000; Anti-AMPK, Cell signaling technology 2532, 1:1000; Anti-p-AMPK, Cell signaling technology 2535, 1:1000; AntiTFEB, Proteintech 13372-1-AP, 1:1000; Anti-mTOR, Proteintech 66888-1-Ig, 1:1000; AntiSTIM1, Proteintech 11565-1-AP, 1:1000; Anti-VAPB, Proteintech 14477-1-AP/66191-1-Ig, 1:1000; Anti-TMEM192, Proteintech 28263-1-AP, 1:1000; Anti-ORAI1, Proteintech 66223-1-Ig, 1:1000; Anti-ULK1, Proteintech 29005-1-AP, 1:1000; Anit-p-ULK1, Proteintech 80218-1-RR, 1:1000).

    Article Title: A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis Pathway
    Article Snippet: The following antibodies and dilutions were used: anti-CHOP/GADD153 1:2000, anti-ATF4 1:2000; anti-XBP-1 1:2000; anti-JNK1 1:5000 (Santa Cruz); anti-caspase-12 1:3000 (Exalpha Biologicals), anti-BAX and anti-BAK 1∶1000 (Upstate Technology); anti-HA 1∶2000 (Roche), anti-caspase-9 1:1000 (Pharmigen), anti-actin 1∶5000 (Chemicon Inc.), anti-SERCA 1∶1000 (Affinity Bioreagents), anti-HSP90 1:1000 (Santa Cruz Biotechnology) and anti-pPERK, and p-JNK 1∶2000 (Cell Signaling).

    Article Title: RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK–CHOP signaling
    Article Snippet: Primary antibodies included anti-RCN1 (Bethyl, Montgomery, TX, USA), anti-GFP (MBL, Nagoya, Japan), anti-Flag (Sigma), anti-IP 3 R1 (Bethyl), anti-tubulin (Sigma), anti-ATF4 (GeneTex, San Antonio, TX, USA), anti-pIRE1 (Abcam, Cambridge, MA, USA), anti-pPERK, anti-caspase-3, anti-PERK, anti-IRE1, anti-GAPDH, anti-CHOP, anti-eIF2α and anti-GRP78/BiP (Cell Signaling Technology, Danvers, MA, USA).

    Article Title: Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice
    Article Snippet: Dilution ratio of primary antibodies used: anti-p-AKT (1:1000; CST, 4058S), anti-AKT (1:1000; CST, 9272S), anti-p-GSK3β (1:1000; Proteintech, 67558-1-Ig), anti-GSK3β (1:1000; Proteintech, 22104-1-AP), anti-p-FOXO1/FOXO3a (1:1000; Beyotime, AF605), anti-FOXO1 (1:1000; Beyotime, AF603), anti-β-actin (1:1000; ZSbio, TA-09), anti-p-IGF-1Rβ/IRβ (1:1000; CST, 3024T), anti-IGF-1Rβ (1:1000; CST, 9750T), anti-p-SRC (1:1000; CST, 6943S), anti-SRC (1:1000; CST, 2109S), anti-p-EGFR (1:1000; CST, 2234S), anti-EGFR (1:1000; CST, 4267S), anti-CAV1 (1:1000; CST, 3267S), anti-CC3 (1:1000; CST, 9661S), anti-CHOP (1:1000; Proteintech, 15204-1-AP), anti-pPERK (1:1000; CST, 3179S), anti-PERK (1:1000; CST, 3192S), anti-β-Tubulin (1:2000; ZSbio, TA-10).

    Article Title: Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling
    Article Snippet: The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and anti-β-arrestin2 antibodies were from Cell Signaling Technology (Boston, MA, USA); anti-E-cadherin, anti-N-cadherin, anti-snai1, and anti-β-actin antibodies were purchased from Santa Cruz (Dallas, TX, USA).

    Article Title: Dual role of sphingosine kinase-1 in promoting the differentiation of dermal fibroblasts and the dissemination of melanoma cells.
    Article Snippet: Despite progress in the understanding of the biology and genetics of melanoma, no effective treatment against this cancer is available.. The adjacent microenvironment has an important role in melanoma progression.. Defining the molecular signals that control the bidirectional dialog between malignant cells and the surrounding stroma is crucial for efficient targeted therapy.

    Article Title: Insulin Secretion and Ca 2+ Dynamics in β-Cells Are Regulated by PERK (EIF2AK3) in Concert with Calcineurin
    Article Snippet: Primary antibodies used in the analysis were: anti-eIF2α-P (1:500, Invitrogen), anti-tubulin (1:1000, Sigma), anti-PERK (1:500, Cell Signaling), anti-pPERK (1:500, Cell Signaling), anti-SERCA N1 (1:5000), and anti-calnexin (1:1000, Enzo Life Sciences).

    Article Title: TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways
    Article Snippet: For anti-BCL-2 (Abcam), anti-BAX (Abcam), and anti-pPERK (rabbit monoclonal antibody; 1:1000, v / v ) (Cell Signaling Technology, Danvers, MA, USA), after overnight incubation, the membranes were washed three times with Tris-buffered saline containing 0.1% ( v / v ) Tween 20 (TBST) for 5 min each, after which they were incubated with secondary antibody [Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488); 1:500 v / v ] for 1 h. After incubation, the membranes were washed three times with TBST buffer and immediately visualized using a ChemiDoc system (Bio-Rad Laboratories).

    Saline:

    Article Title: The p53 target DRAM1 modulates calcium homeostasis and ER stress by promoting contact between lysosomes and the ER through STIM1
    Article Snippet: Following blocking with 5% nonfat milk at room temperature for 1 h, membranes were incubated with following primary antibodies at 4°C overnight (Anti-DRAM1, Abclonal A15468, 1:1000; Anti-ACTB, Proteintech, 1:3000; Anti-Bax, Santa Cruz sc-526, 1:300; Anti-P53, Santa Cruz sc-6243, 1:300; Anti-Beclin1, Proteintech 11306-1-AP, 1:1000; Anti-CHOP, Proteintech 15204-1-AP, 1:1000; Anti-GRP78, Proteintech 11587-1-AP, 1:1000; Anti-LC3, Proteintech 14600-1-AP, 1:1000; Anti-P62, Proteintech 18420-1-AP, 1:1000; Anti-ATF4, Proteintech 10835-1-AP, 1:1000; Anti-EIF2α, Proteintech 11233-1-AP, 1:1000; Anti-p-EIF2α, Cell signaling technology 9721, 1:1000; Anti-pPERK, Cell signaling technology 3197, 1:1000; Anti-Calnexin, Proteintech 10427-2-AP, 1:1000; Anti-Calreticulin, Proteintech 27298-1-AP, 1:1000; Anti-KTN1, Proteintech 19841-1-AP, 1:1000; Anti-Ribophorin, Proteintech 12894-1-AP, 1:1000; Anti-C53, Proteintech 11007-1-AP, 1:1000; Anti-ZMPSTE24, Proteintech 12847-1-AP, 1:1000; Anti-FAM134, Proteintech 21537-1-AP, 1:1000; Anti-TEX264, Proteintech 25858-1-AP, 1:1000; Anti-CKAP4, Proteintech 16686-1-AP, 1:1000; Anti-Sec62, Proteintech 28693-1-AP, 1:1000; Anti-GP78, Proteintech 16675-1-AP, 1:1000; Anti-ATG10, Prosci 4399, 1:1000; Anti-ATG16, Prosci 4425, 1:1000; Anti-LAMP2, Prosci 3627, 1:1000; Anti-p-4E-BP1, Cell signaling technology 13443, 1:1000; Anti-AMPK, Cell signaling technology 2532, 1:1000; Anti-p-AMPK, Cell signaling technology 2535, 1:1000; AntiTFEB, Proteintech 13372-1-AP, 1:1000; Anti-mTOR, Proteintech 66888-1-Ig, 1:1000; AntiSTIM1, Proteintech 11565-1-AP, 1:1000; Anti-VAPB, Proteintech 14477-1-AP/66191-1-Ig, 1:1000; Anti-TMEM192, Proteintech 28263-1-AP, 1:1000; Anti-ORAI1, Proteintech 66223-1-Ig, 1:1000; Anti-ULK1, Proteintech 29005-1-AP, 1:1000; Anit-p-ULK1, Proteintech 80218-1-RR, 1:1000).

    Article Title: A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis Pathway
    Article Snippet: The following antibodies and dilutions were used: anti-CHOP/GADD153 1:2000, anti-ATF4 1:2000; anti-XBP-1 1:2000; anti-JNK1 1:5000 (Santa Cruz); anti-caspase-12 1:3000 (Exalpha Biologicals), anti-BAX and anti-BAK 1∶1000 (Upstate Technology); anti-HA 1∶2000 (Roche), anti-caspase-9 1:1000 (Pharmigen), anti-actin 1∶5000 (Chemicon Inc.), anti-SERCA 1∶1000 (Affinity Bioreagents), anti-HSP90 1:1000 (Santa Cruz Biotechnology) and anti-pPERK, and p-JNK 1∶2000 (Cell Signaling).

    Article Title: RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK–CHOP signaling
    Article Snippet: Primary antibodies included anti-RCN1 (Bethyl, Montgomery, TX, USA), anti-GFP (MBL, Nagoya, Japan), anti-Flag (Sigma), anti-IP 3 R1 (Bethyl), anti-tubulin (Sigma), anti-ATF4 (GeneTex, San Antonio, TX, USA), anti-pIRE1 (Abcam, Cambridge, MA, USA), anti-pPERK, anti-caspase-3, anti-PERK, anti-IRE1, anti-GAPDH, anti-CHOP, anti-eIF2α and anti-GRP78/BiP (Cell Signaling Technology, Danvers, MA, USA).

    Article Title: Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice
    Article Snippet: Dilution ratio of primary antibodies used: anti-p-AKT (1:1000; CST, 4058S), anti-AKT (1:1000; CST, 9272S), anti-p-GSK3β (1:1000; Proteintech, 67558-1-Ig), anti-GSK3β (1:1000; Proteintech, 22104-1-AP), anti-p-FOXO1/FOXO3a (1:1000; Beyotime, AF605), anti-FOXO1 (1:1000; Beyotime, AF603), anti-β-actin (1:1000; ZSbio, TA-09), anti-p-IGF-1Rβ/IRβ (1:1000; CST, 3024T), anti-IGF-1Rβ (1:1000; CST, 9750T), anti-p-SRC (1:1000; CST, 6943S), anti-SRC (1:1000; CST, 2109S), anti-p-EGFR (1:1000; CST, 2234S), anti-EGFR (1:1000; CST, 4267S), anti-CAV1 (1:1000; CST, 3267S), anti-CC3 (1:1000; CST, 9661S), anti-CHOP (1:1000; Proteintech, 15204-1-AP), anti-pPERK (1:1000; CST, 3179S), anti-PERK (1:1000; CST, 3192S), anti-β-Tubulin (1:2000; ZSbio, TA-10).

    Article Title: Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling
    Article Snippet: The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and anti-β-arrestin2 antibodies were from Cell Signaling Technology (Boston, MA, USA); anti-E-cadherin, anti-N-cadherin, anti-snai1, and anti-β-actin antibodies were purchased from Santa Cruz (Dallas, TX, USA).

    Article Title: Dual role of sphingosine kinase-1 in promoting the differentiation of dermal fibroblasts and the dissemination of melanoma cells.
    Article Snippet: Despite progress in the understanding of the biology and genetics of melanoma, no effective treatment against this cancer is available.. The adjacent microenvironment has an important role in melanoma progression.. Defining the molecular signals that control the bidirectional dialog between malignant cells and the surrounding stroma is crucial for efficient targeted therapy.

    Article Title: Insulin Secretion and Ca 2+ Dynamics in β-Cells Are Regulated by PERK (EIF2AK3) in Concert with Calcineurin
    Article Snippet: Primary antibodies used in the analysis were: anti-eIF2α-P (1:500, Invitrogen), anti-tubulin (1:1000, Sigma), anti-PERK (1:500, Cell Signaling), anti-pPERK (1:500, Cell Signaling), anti-SERCA N1 (1:5000), and anti-calnexin (1:1000, Enzo Life Sciences).

    Article Title: TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways
    Article Snippet: For anti-BCL-2 (Abcam), anti-BAX (Abcam), and anti-pPERK (rabbit monoclonal antibody; 1:1000, v / v ) (Cell Signaling Technology, Danvers, MA, USA), after overnight incubation, the membranes were washed three times with Tris-buffered saline containing 0.1% ( v / v ) Tween 20 (TBST) for 5 min each, after which they were incubated with secondary antibody [Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488); 1:500 v / v ] for 1 h. After incubation, the membranes were washed three times with TBST buffer and immediately visualized using a ChemiDoc system (Bio-Rad Laboratories).

    Transfection:

    Article Title: The p53 target DRAM1 modulates calcium homeostasis and ER stress by promoting contact between lysosomes and the ER through STIM1
    Article Snippet: Following blocking with 5% nonfat milk at room temperature for 1 h, membranes were incubated with following primary antibodies at 4°C overnight (Anti-DRAM1, Abclonal A15468, 1:1000; Anti-ACTB, Proteintech, 1:3000; Anti-Bax, Santa Cruz sc-526, 1:300; Anti-P53, Santa Cruz sc-6243, 1:300; Anti-Beclin1, Proteintech 11306-1-AP, 1:1000; Anti-CHOP, Proteintech 15204-1-AP, 1:1000; Anti-GRP78, Proteintech 11587-1-AP, 1:1000; Anti-LC3, Proteintech 14600-1-AP, 1:1000; Anti-P62, Proteintech 18420-1-AP, 1:1000; Anti-ATF4, Proteintech 10835-1-AP, 1:1000; Anti-EIF2α, Proteintech 11233-1-AP, 1:1000; Anti-p-EIF2α, Cell signaling technology 9721, 1:1000; Anti-pPERK, Cell signaling technology 3197, 1:1000; Anti-Calnexin, Proteintech 10427-2-AP, 1:1000; Anti-Calreticulin, Proteintech 27298-1-AP, 1:1000; Anti-KTN1, Proteintech 19841-1-AP, 1:1000; Anti-Ribophorin, Proteintech 12894-1-AP, 1:1000; Anti-C53, Proteintech 11007-1-AP, 1:1000; Anti-ZMPSTE24, Proteintech 12847-1-AP, 1:1000; Anti-FAM134, Proteintech 21537-1-AP, 1:1000; Anti-TEX264, Proteintech 25858-1-AP, 1:1000; Anti-CKAP4, Proteintech 16686-1-AP, 1:1000; Anti-Sec62, Proteintech 28693-1-AP, 1:1000; Anti-GP78, Proteintech 16675-1-AP, 1:1000; Anti-ATG10, Prosci 4399, 1:1000; Anti-ATG16, Prosci 4425, 1:1000; Anti-LAMP2, Prosci 3627, 1:1000; Anti-p-4E-BP1, Cell signaling technology 13443, 1:1000; Anti-AMPK, Cell signaling technology 2532, 1:1000; Anti-p-AMPK, Cell signaling technology 2535, 1:1000; AntiTFEB, Proteintech 13372-1-AP, 1:1000; Anti-mTOR, Proteintech 66888-1-Ig, 1:1000; AntiSTIM1, Proteintech 11565-1-AP, 1:1000; Anti-VAPB, Proteintech 14477-1-AP/66191-1-Ig, 1:1000; Anti-TMEM192, Proteintech 28263-1-AP, 1:1000; Anti-ORAI1, Proteintech 66223-1-Ig, 1:1000; Anti-ULK1, Proteintech 29005-1-AP, 1:1000; Anit-p-ULK1, Proteintech 80218-1-RR, 1:1000).

    Article Title: A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis Pathway
    Article Snippet: The following antibodies and dilutions were used: anti-CHOP/GADD153 1:2000, anti-ATF4 1:2000; anti-XBP-1 1:2000; anti-JNK1 1:5000 (Santa Cruz); anti-caspase-12 1:3000 (Exalpha Biologicals), anti-BAX and anti-BAK 1∶1000 (Upstate Technology); anti-HA 1∶2000 (Roche), anti-caspase-9 1:1000 (Pharmigen), anti-actin 1∶5000 (Chemicon Inc.), anti-SERCA 1∶1000 (Affinity Bioreagents), anti-HSP90 1:1000 (Santa Cruz Biotechnology) and anti-pPERK, and p-JNK 1∶2000 (Cell Signaling).

    Article Title: RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK–CHOP signaling
    Article Snippet: Primary antibodies included anti-RCN1 (Bethyl, Montgomery, TX, USA), anti-GFP (MBL, Nagoya, Japan), anti-Flag (Sigma), anti-IP 3 R1 (Bethyl), anti-tubulin (Sigma), anti-ATF4 (GeneTex, San Antonio, TX, USA), anti-pIRE1 (Abcam, Cambridge, MA, USA), anti-pPERK, anti-caspase-3, anti-PERK, anti-IRE1, anti-GAPDH, anti-CHOP, anti-eIF2α and anti-GRP78/BiP (Cell Signaling Technology, Danvers, MA, USA).

    Article Title: Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice
    Article Snippet: Dilution ratio of primary antibodies used: anti-p-AKT (1:1000; CST, 4058S), anti-AKT (1:1000; CST, 9272S), anti-p-GSK3β (1:1000; Proteintech, 67558-1-Ig), anti-GSK3β (1:1000; Proteintech, 22104-1-AP), anti-p-FOXO1/FOXO3a (1:1000; Beyotime, AF605), anti-FOXO1 (1:1000; Beyotime, AF603), anti-β-actin (1:1000; ZSbio, TA-09), anti-p-IGF-1Rβ/IRβ (1:1000; CST, 3024T), anti-IGF-1Rβ (1:1000; CST, 9750T), anti-p-SRC (1:1000; CST, 6943S), anti-SRC (1:1000; CST, 2109S), anti-p-EGFR (1:1000; CST, 2234S), anti-EGFR (1:1000; CST, 4267S), anti-CAV1 (1:1000; CST, 3267S), anti-CC3 (1:1000; CST, 9661S), anti-CHOP (1:1000; Proteintech, 15204-1-AP), anti-pPERK (1:1000; CST, 3179S), anti-PERK (1:1000; CST, 3192S), anti-β-Tubulin (1:2000; ZSbio, TA-10).

    Article Title: Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling
    Article Snippet: The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and anti-β-arrestin2 antibodies were from Cell Signaling Technology (Boston, MA, USA); anti-E-cadherin, anti-N-cadherin, anti-snai1, and anti-β-actin antibodies were purchased from Santa Cruz (Dallas, TX, USA).

    Article Title: Dual role of sphingosine kinase-1 in promoting the differentiation of dermal fibroblasts and the dissemination of melanoma cells.
    Article Snippet: Despite progress in the understanding of the biology and genetics of melanoma, no effective treatment against this cancer is available.. The adjacent microenvironment has an important role in melanoma progression.. Defining the molecular signals that control the bidirectional dialog between malignant cells and the surrounding stroma is crucial for efficient targeted therapy.

    Article Title: Insulin Secretion and Ca 2+ Dynamics in β-Cells Are Regulated by PERK (EIF2AK3) in Concert with Calcineurin
    Article Snippet: Primary antibodies used in the analysis were: anti-eIF2α-P (1:500, Invitrogen), anti-tubulin (1:1000, Sigma), anti-PERK (1:500, Cell Signaling), anti-pPERK (1:500, Cell Signaling), anti-SERCA N1 (1:5000), and anti-calnexin (1:1000, Enzo Life Sciences).

    Article Title: TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways
    Article Snippet: For anti-BCL-2 (Abcam), anti-BAX (Abcam), and anti-pPERK (rabbit monoclonal antibody; 1:1000, v / v ) (Cell Signaling Technology, Danvers, MA, USA), after overnight incubation, the membranes were washed three times with Tris-buffered saline containing 0.1% ( v / v ) Tween 20 (TBST) for 5 min each, after which they were incubated with secondary antibody [Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488); 1:500 v / v ] for 1 h. After incubation, the membranes were washed three times with TBST buffer and immediately visualized using a ChemiDoc system (Bio-Rad Laboratories).

    Plasmid Preparation:

    Article Title: The p53 target DRAM1 modulates calcium homeostasis and ER stress by promoting contact between lysosomes and the ER through STIM1
    Article Snippet: Following blocking with 5% nonfat milk at room temperature for 1 h, membranes were incubated with following primary antibodies at 4°C overnight (Anti-DRAM1, Abclonal A15468, 1:1000; Anti-ACTB, Proteintech, 1:3000; Anti-Bax, Santa Cruz sc-526, 1:300; Anti-P53, Santa Cruz sc-6243, 1:300; Anti-Beclin1, Proteintech 11306-1-AP, 1:1000; Anti-CHOP, Proteintech 15204-1-AP, 1:1000; Anti-GRP78, Proteintech 11587-1-AP, 1:1000; Anti-LC3, Proteintech 14600-1-AP, 1:1000; Anti-P62, Proteintech 18420-1-AP, 1:1000; Anti-ATF4, Proteintech 10835-1-AP, 1:1000; Anti-EIF2α, Proteintech 11233-1-AP, 1:1000; Anti-p-EIF2α, Cell signaling technology 9721, 1:1000; Anti-pPERK, Cell signaling technology 3197, 1:1000; Anti-Calnexin, Proteintech 10427-2-AP, 1:1000; Anti-Calreticulin, Proteintech 27298-1-AP, 1:1000; Anti-KTN1, Proteintech 19841-1-AP, 1:1000; Anti-Ribophorin, Proteintech 12894-1-AP, 1:1000; Anti-C53, Proteintech 11007-1-AP, 1:1000; Anti-ZMPSTE24, Proteintech 12847-1-AP, 1:1000; Anti-FAM134, Proteintech 21537-1-AP, 1:1000; Anti-TEX264, Proteintech 25858-1-AP, 1:1000; Anti-CKAP4, Proteintech 16686-1-AP, 1:1000; Anti-Sec62, Proteintech 28693-1-AP, 1:1000; Anti-GP78, Proteintech 16675-1-AP, 1:1000; Anti-ATG10, Prosci 4399, 1:1000; Anti-ATG16, Prosci 4425, 1:1000; Anti-LAMP2, Prosci 3627, 1:1000; Anti-p-4E-BP1, Cell signaling technology 13443, 1:1000; Anti-AMPK, Cell signaling technology 2532, 1:1000; Anti-p-AMPK, Cell signaling technology 2535, 1:1000; AntiTFEB, Proteintech 13372-1-AP, 1:1000; Anti-mTOR, Proteintech 66888-1-Ig, 1:1000; AntiSTIM1, Proteintech 11565-1-AP, 1:1000; Anti-VAPB, Proteintech 14477-1-AP/66191-1-Ig, 1:1000; Anti-TMEM192, Proteintech 28263-1-AP, 1:1000; Anti-ORAI1, Proteintech 66223-1-Ig, 1:1000; Anti-ULK1, Proteintech 29005-1-AP, 1:1000; Anit-p-ULK1, Proteintech 80218-1-RR, 1:1000).

    Article Title: A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis Pathway
    Article Snippet: The following antibodies and dilutions were used: anti-CHOP/GADD153 1:2000, anti-ATF4 1:2000; anti-XBP-1 1:2000; anti-JNK1 1:5000 (Santa Cruz); anti-caspase-12 1:3000 (Exalpha Biologicals), anti-BAX and anti-BAK 1∶1000 (Upstate Technology); anti-HA 1∶2000 (Roche), anti-caspase-9 1:1000 (Pharmigen), anti-actin 1∶5000 (Chemicon Inc.), anti-SERCA 1∶1000 (Affinity Bioreagents), anti-HSP90 1:1000 (Santa Cruz Biotechnology) and anti-pPERK, and p-JNK 1∶2000 (Cell Signaling).

    Article Title: RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK–CHOP signaling
    Article Snippet: Primary antibodies included anti-RCN1 (Bethyl, Montgomery, TX, USA), anti-GFP (MBL, Nagoya, Japan), anti-Flag (Sigma), anti-IP 3 R1 (Bethyl), anti-tubulin (Sigma), anti-ATF4 (GeneTex, San Antonio, TX, USA), anti-pIRE1 (Abcam, Cambridge, MA, USA), anti-pPERK, anti-caspase-3, anti-PERK, anti-IRE1, anti-GAPDH, anti-CHOP, anti-eIF2α and anti-GRP78/BiP (Cell Signaling Technology, Danvers, MA, USA).

    Article Title: Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice
    Article Snippet: Dilution ratio of primary antibodies used: anti-p-AKT (1:1000; CST, 4058S), anti-AKT (1:1000; CST, 9272S), anti-p-GSK3β (1:1000; Proteintech, 67558-1-Ig), anti-GSK3β (1:1000; Proteintech, 22104-1-AP), anti-p-FOXO1/FOXO3a (1:1000; Beyotime, AF605), anti-FOXO1 (1:1000; Beyotime, AF603), anti-β-actin (1:1000; ZSbio, TA-09), anti-p-IGF-1Rβ/IRβ (1:1000; CST, 3024T), anti-IGF-1Rβ (1:1000; CST, 9750T), anti-p-SRC (1:1000; CST, 6943S), anti-SRC (1:1000; CST, 2109S), anti-p-EGFR (1:1000; CST, 2234S), anti-EGFR (1:1000; CST, 4267S), anti-CAV1 (1:1000; CST, 3267S), anti-CC3 (1:1000; CST, 9661S), anti-CHOP (1:1000; Proteintech, 15204-1-AP), anti-pPERK (1:1000; CST, 3179S), anti-PERK (1:1000; CST, 3192S), anti-β-Tubulin (1:2000; ZSbio, TA-10).

    Article Title: Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling
    Article Snippet: The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and anti-β-arrestin2 antibodies were from Cell Signaling Technology (Boston, MA, USA); anti-E-cadherin, anti-N-cadherin, anti-snai1, and anti-β-actin antibodies were purchased from Santa Cruz (Dallas, TX, USA).

    Article Title: Dual role of sphingosine kinase-1 in promoting the differentiation of dermal fibroblasts and the dissemination of melanoma cells.
    Article Snippet: Despite progress in the understanding of the biology and genetics of melanoma, no effective treatment against this cancer is available.. The adjacent microenvironment has an important role in melanoma progression.. Defining the molecular signals that control the bidirectional dialog between malignant cells and the surrounding stroma is crucial for efficient targeted therapy.

    Article Title: Insulin Secretion and Ca 2+ Dynamics in β-Cells Are Regulated by PERK (EIF2AK3) in Concert with Calcineurin
    Article Snippet: Primary antibodies used in the analysis were: anti-eIF2α-P (1:500, Invitrogen), anti-tubulin (1:1000, Sigma), anti-PERK (1:500, Cell Signaling), anti-pPERK (1:500, Cell Signaling), anti-SERCA N1 (1:5000), and anti-calnexin (1:1000, Enzo Life Sciences).

    Article Title: TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways
    Article Snippet: For anti-BCL-2 (Abcam), anti-BAX (Abcam), and anti-pPERK (rabbit monoclonal antibody; 1:1000, v / v ) (Cell Signaling Technology, Danvers, MA, USA), after overnight incubation, the membranes were washed three times with Tris-buffered saline containing 0.1% ( v / v ) Tween 20 (TBST) for 5 min each, after which they were incubated with secondary antibody [Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488); 1:500 v / v ] for 1 h. After incubation, the membranes were washed three times with TBST buffer and immediately visualized using a ChemiDoc system (Bio-Rad Laboratories).



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    SB supplement ameliorated TMAO-mediated activation of ER stress signaling and dysregulation of ionic signaling. (A) and (B) Representative images and statistical data show the expression of <t>pPERK,</t> <t>PERK,</t> <t>pIRE1α,</t> IRE1α, pNF-κB, NF-κB, pIP3R, IP3R, NCX, Kv1.5, and β-actin in HL-1 cells from control group (n = 3), TMAO treated (n = 3), SB treated (n = 3), and TMAO combined with SB treated group (n = 3) in three independent experiments. β-Actin was used as a loading control. * P < 0.05. ** P < 0.01. TMAO versus control or SB or TMAO combined with SB treatment.
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    Image Search Results


    SB supplement ameliorated TMAO-mediated activation of ER stress signaling and dysregulation of ionic signaling. (A) and (B) Representative images and statistical data show the expression of pPERK, PERK, pIRE1α, IRE1α, pNF-κB, NF-κB, pIP3R, IP3R, NCX, Kv1.5, and β-actin in HL-1 cells from control group (n = 3), TMAO treated (n = 3), SB treated (n = 3), and TMAO combined with SB treated group (n = 3) in three independent experiments. β-Actin was used as a loading control. * P < 0.05. ** P < 0.01. TMAO versus control or SB or TMAO combined with SB treatment.

    Journal: Journal of Advanced Research

    Article Title: Short-chain fatty acid butyrate against TMAO activating endoplasmic-reticulum stress and PERK/IRE1-axis with reducing atrial arrhythmia

    doi: 10.1016/j.jare.2024.08.009

    Figure Lengend Snippet: SB supplement ameliorated TMAO-mediated activation of ER stress signaling and dysregulation of ionic signaling. (A) and (B) Representative images and statistical data show the expression of pPERK, PERK, pIRE1α, IRE1α, pNF-κB, NF-κB, pIP3R, IP3R, NCX, Kv1.5, and β-actin in HL-1 cells from control group (n = 3), TMAO treated (n = 3), SB treated (n = 3), and TMAO combined with SB treated group (n = 3) in three independent experiments. β-Actin was used as a loading control. * P < 0.05. ** P < 0.01. TMAO versus control or SB or TMAO combined with SB treatment.

    Article Snippet: Additionally, the atrial sections were incubated with primary antibodies against pPERK (#3179, Cell Signaling Technology), pIRE1α (NB100-2323, Novus), and pNF-κB (ab86299, Abcam).

    Techniques: Activation Assay, Expressing, Control

    SB supplement ameliorated TMAO-mediated overexpression of ER stress signaling and pNF-κB in HL-1 cells. (A) and (B) show the representative images and statistical data of pPERK, pIRE1α, pNF-κB, and ER marker-Calnexin expression in HL-1 cells through cellular immunostaing assay in control group (n = 3), TMAO treated (n = 3), SB treated (n = 3), and TMAO combined with SB treated group (n = 3) in three independent experiments. (bar, 25 μm). ** P < 0.01. TMAO versus control or SB or TMAO combined with SB treatment.

    Journal: Journal of Advanced Research

    Article Title: Short-chain fatty acid butyrate against TMAO activating endoplasmic-reticulum stress and PERK/IRE1-axis with reducing atrial arrhythmia

    doi: 10.1016/j.jare.2024.08.009

    Figure Lengend Snippet: SB supplement ameliorated TMAO-mediated overexpression of ER stress signaling and pNF-κB in HL-1 cells. (A) and (B) show the representative images and statistical data of pPERK, pIRE1α, pNF-κB, and ER marker-Calnexin expression in HL-1 cells through cellular immunostaing assay in control group (n = 3), TMAO treated (n = 3), SB treated (n = 3), and TMAO combined with SB treated group (n = 3) in three independent experiments. (bar, 25 μm). ** P < 0.01. TMAO versus control or SB or TMAO combined with SB treatment.

    Article Snippet: Additionally, the atrial sections were incubated with primary antibodies against pPERK (#3179, Cell Signaling Technology), pIRE1α (NB100-2323, Novus), and pNF-κB (ab86299, Abcam).

    Techniques: Over Expression, Marker, Expressing, Control

    SB supplement ameliorated atrial fibrosis and PERK/IRE1α/NF-κB axis of TMAO-treated mice. The expression of atrial fibrosis (blue area) was detected by Masson's staining and immunostaining of pPERK, pIRE1α, and pNF-κB in the atrium tissues derived from mice gavage with control (n = 5), TMAO (n = 5), and TMAO combined with SB (n = 5). The expressions of pPERK are indicated by hollow arrowheads. (bar, 25 μm). ** p < 0.01. TMAO treatment versus control group or TMAO combined with SB treated group. (For interpretation of the references to colour in this Fig. legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Short-chain fatty acid butyrate against TMAO activating endoplasmic-reticulum stress and PERK/IRE1-axis with reducing atrial arrhythmia

    doi: 10.1016/j.jare.2024.08.009

    Figure Lengend Snippet: SB supplement ameliorated atrial fibrosis and PERK/IRE1α/NF-κB axis of TMAO-treated mice. The expression of atrial fibrosis (blue area) was detected by Masson's staining and immunostaining of pPERK, pIRE1α, and pNF-κB in the atrium tissues derived from mice gavage with control (n = 5), TMAO (n = 5), and TMAO combined with SB (n = 5). The expressions of pPERK are indicated by hollow arrowheads. (bar, 25 μm). ** p < 0.01. TMAO treatment versus control group or TMAO combined with SB treated group. (For interpretation of the references to colour in this Fig. legend, the reader is referred to the web version of this article.)

    Article Snippet: Additionally, the atrial sections were incubated with primary antibodies against pPERK (#3179, Cell Signaling Technology), pIRE1α (NB100-2323, Novus), and pNF-κB (ab86299, Abcam).

    Techniques: Expressing, Staining, Immunostaining, Derivative Assay, Control