anti pperk (Cell Signaling Technology Inc)
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Anti Pperk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-pperk/pm41596608-269-10-18
Average 86 stars, based on 1 article reviews
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Blocking Assay:Article Title: The p53 target DRAM1 modulates calcium homeostasis and ER stress by promoting contact between lysosomes and the ER through STIM1 Article Snippet: Following blocking with 5% nonfat milk at room temperature for 1 h, membranes were incubated with following primary antibodies at 4°C overnight (Anti-DRAM1, Abclonal A15468, 1:1000; Anti-ACTB, Proteintech, 1:3000; Anti-Bax, Santa Cruz sc-526, 1:300; Anti-P53, Santa Cruz sc-6243, 1:300; Anti-Beclin1, Proteintech 11306-1-AP, 1:1000; Anti-CHOP, Proteintech 15204-1-AP, 1:1000; Anti-GRP78, Proteintech 11587-1-AP, 1:1000; Anti-LC3, Proteintech 14600-1-AP, 1:1000; Anti-P62, Proteintech 18420-1-AP, 1:1000; Anti-ATF4, Proteintech 10835-1-AP, 1:1000; Anti-EIF2α, Proteintech 11233-1-AP, 1:1000; Anti-p-EIF2α, Article Title: A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis Pathway Article Snippet: The following antibodies and dilutions were used: anti-CHOP/GADD153 1:2000, anti-ATF4 1:2000; anti-XBP-1 1:2000; anti-JNK1 1:5000 (Santa Cruz); anti-caspase-12 1:3000 (Exalpha Biologicals), anti-BAX and anti-BAK 1∶1000 (Upstate Technology); anti-HA 1∶2000 (Roche), anti-caspase-9 1:1000 (Pharmigen), anti-actin 1∶5000 (Chemicon Inc.), anti-SERCA 1∶1000 (Affinity Bioreagents), anti-HSP90 1:1000 (Santa Cruz Biotechnology) and anti-pPERK, and Article Title: RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK–CHOP signaling Article Snippet: Primary antibodies included anti-RCN1 (Bethyl, Montgomery, TX, USA), anti-GFP (MBL, Nagoya, Japan), anti-Flag (Sigma), anti-IP 3 R1 (Bethyl), anti-tubulin (Sigma), anti-ATF4 (GeneTex, San Antonio, TX, USA), anti-pIRE1 (Abcam, Cambridge, MA, USA), anti-pPERK, anti-caspase-3, anti-PERK, anti-IRE1, anti-GAPDH, anti-CHOP, anti-eIF2α and Article Title: Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice Article Snippet: Dilution ratio of primary antibodies used: Article Title: Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling Article Snippet: The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and Article Title: Dual role of sphingosine kinase-1 in promoting the differentiation of dermal fibroblasts and the dissemination of melanoma cells. Article Snippet: Despite progress in the understanding of the biology and genetics of melanoma, no effective treatment against this cancer is available.. The adjacent microenvironment has an important role in melanoma progression.. Defining the molecular signals that control the bidirectional dialog between malignant cells and the surrounding stroma is crucial for efficient targeted therapy. Article Title: Insulin Secretion and Ca 2+ Dynamics in β-Cells Are Regulated by PERK (EIF2AK3) in Concert with Calcineurin Article Snippet: Primary antibodies used in the analysis were: anti-eIF2α-P (1:500, Invitrogen), anti-tubulin (1:1000, Sigma), Article Title: TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways Article Snippet: For anti-BCL-2 (Abcam), anti-BAX (Abcam), and Incubation:Article Title: The p53 target DRAM1 modulates calcium homeostasis and ER stress by promoting contact between lysosomes and the ER through STIM1 Article Snippet: Following blocking with 5% nonfat milk at room temperature for 1 h, membranes were incubated with following primary antibodies at 4°C overnight (Anti-DRAM1, Abclonal A15468, 1:1000; Anti-ACTB, Proteintech, 1:3000; Anti-Bax, Santa Cruz sc-526, 1:300; Anti-P53, Santa Cruz sc-6243, 1:300; Anti-Beclin1, Proteintech 11306-1-AP, 1:1000; Anti-CHOP, Proteintech 15204-1-AP, 1:1000; Anti-GRP78, Proteintech 11587-1-AP, 1:1000; Anti-LC3, Proteintech 14600-1-AP, 1:1000; Anti-P62, Proteintech 18420-1-AP, 1:1000; Anti-ATF4, Proteintech 10835-1-AP, 1:1000; Anti-EIF2α, Proteintech 11233-1-AP, 1:1000; Anti-p-EIF2α, Article Title: A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis Pathway Article Snippet: The following antibodies and dilutions were used: anti-CHOP/GADD153 1:2000, anti-ATF4 1:2000; anti-XBP-1 1:2000; anti-JNK1 1:5000 (Santa Cruz); anti-caspase-12 1:3000 (Exalpha Biologicals), anti-BAX and anti-BAK 1∶1000 (Upstate Technology); anti-HA 1∶2000 (Roche), anti-caspase-9 1:1000 (Pharmigen), anti-actin 1∶5000 (Chemicon Inc.), anti-SERCA 1∶1000 (Affinity Bioreagents), anti-HSP90 1:1000 (Santa Cruz Biotechnology) and anti-pPERK, and Article Title: RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK–CHOP signaling Article Snippet: Primary antibodies included anti-RCN1 (Bethyl, Montgomery, TX, USA), anti-GFP (MBL, Nagoya, Japan), anti-Flag (Sigma), anti-IP 3 R1 (Bethyl), anti-tubulin (Sigma), anti-ATF4 (GeneTex, San Antonio, TX, USA), anti-pIRE1 (Abcam, Cambridge, MA, USA), anti-pPERK, anti-caspase-3, anti-PERK, anti-IRE1, anti-GAPDH, anti-CHOP, anti-eIF2α and Article Title: Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice Article Snippet: Dilution ratio of primary antibodies used: Article Title: Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling Article Snippet: The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and Article Title: Dual role of sphingosine kinase-1 in promoting the differentiation of dermal fibroblasts and the dissemination of melanoma cells. Article Snippet: Despite progress in the understanding of the biology and genetics of melanoma, no effective treatment against this cancer is available.. The adjacent microenvironment has an important role in melanoma progression.. Defining the molecular signals that control the bidirectional dialog between malignant cells and the surrounding stroma is crucial for efficient targeted therapy. Article Title: Insulin Secretion and Ca 2+ Dynamics in β-Cells Are Regulated by PERK (EIF2AK3) in Concert with Calcineurin Article Snippet: Primary antibodies used in the analysis were: anti-eIF2α-P (1:500, Invitrogen), anti-tubulin (1:1000, Sigma), Article Title: TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways Article Snippet: For anti-BCL-2 (Abcam), anti-BAX (Abcam), and Saline:Article Title: The p53 target DRAM1 modulates calcium homeostasis and ER stress by promoting contact between lysosomes and the ER through STIM1 Article Snippet: Following blocking with 5% nonfat milk at room temperature for 1 h, membranes were incubated with following primary antibodies at 4°C overnight (Anti-DRAM1, Abclonal A15468, 1:1000; Anti-ACTB, Proteintech, 1:3000; Anti-Bax, Santa Cruz sc-526, 1:300; Anti-P53, Santa Cruz sc-6243, 1:300; Anti-Beclin1, Proteintech 11306-1-AP, 1:1000; Anti-CHOP, Proteintech 15204-1-AP, 1:1000; Anti-GRP78, Proteintech 11587-1-AP, 1:1000; Anti-LC3, Proteintech 14600-1-AP, 1:1000; Anti-P62, Proteintech 18420-1-AP, 1:1000; Anti-ATF4, Proteintech 10835-1-AP, 1:1000; Anti-EIF2α, Proteintech 11233-1-AP, 1:1000; Anti-p-EIF2α, Article Title: A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis Pathway Article Snippet: The following antibodies and dilutions were used: anti-CHOP/GADD153 1:2000, anti-ATF4 1:2000; anti-XBP-1 1:2000; anti-JNK1 1:5000 (Santa Cruz); anti-caspase-12 1:3000 (Exalpha Biologicals), anti-BAX and anti-BAK 1∶1000 (Upstate Technology); anti-HA 1∶2000 (Roche), anti-caspase-9 1:1000 (Pharmigen), anti-actin 1∶5000 (Chemicon Inc.), anti-SERCA 1∶1000 (Affinity Bioreagents), anti-HSP90 1:1000 (Santa Cruz Biotechnology) and anti-pPERK, and Article Title: RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK–CHOP signaling Article Snippet: Primary antibodies included anti-RCN1 (Bethyl, Montgomery, TX, USA), anti-GFP (MBL, Nagoya, Japan), anti-Flag (Sigma), anti-IP 3 R1 (Bethyl), anti-tubulin (Sigma), anti-ATF4 (GeneTex, San Antonio, TX, USA), anti-pIRE1 (Abcam, Cambridge, MA, USA), anti-pPERK, anti-caspase-3, anti-PERK, anti-IRE1, anti-GAPDH, anti-CHOP, anti-eIF2α and Article Title: Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice Article Snippet: Dilution ratio of primary antibodies used: Article Title: Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling Article Snippet: The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and Article Title: Dual role of sphingosine kinase-1 in promoting the differentiation of dermal fibroblasts and the dissemination of melanoma cells. Article Snippet: Despite progress in the understanding of the biology and genetics of melanoma, no effective treatment against this cancer is available.. The adjacent microenvironment has an important role in melanoma progression.. Defining the molecular signals that control the bidirectional dialog between malignant cells and the surrounding stroma is crucial for efficient targeted therapy. Article Title: Insulin Secretion and Ca 2+ Dynamics in β-Cells Are Regulated by PERK (EIF2AK3) in Concert with Calcineurin Article Snippet: Primary antibodies used in the analysis were: anti-eIF2α-P (1:500, Invitrogen), anti-tubulin (1:1000, Sigma), Article Title: TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways Article Snippet: For anti-BCL-2 (Abcam), anti-BAX (Abcam), and Transfection:Article Title: The p53 target DRAM1 modulates calcium homeostasis and ER stress by promoting contact between lysosomes and the ER through STIM1 Article Snippet: Following blocking with 5% nonfat milk at room temperature for 1 h, membranes were incubated with following primary antibodies at 4°C overnight (Anti-DRAM1, Abclonal A15468, 1:1000; Anti-ACTB, Proteintech, 1:3000; Anti-Bax, Santa Cruz sc-526, 1:300; Anti-P53, Santa Cruz sc-6243, 1:300; Anti-Beclin1, Proteintech 11306-1-AP, 1:1000; Anti-CHOP, Proteintech 15204-1-AP, 1:1000; Anti-GRP78, Proteintech 11587-1-AP, 1:1000; Anti-LC3, Proteintech 14600-1-AP, 1:1000; Anti-P62, Proteintech 18420-1-AP, 1:1000; Anti-ATF4, Proteintech 10835-1-AP, 1:1000; Anti-EIF2α, Proteintech 11233-1-AP, 1:1000; Anti-p-EIF2α, Article Title: A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis Pathway Article Snippet: The following antibodies and dilutions were used: anti-CHOP/GADD153 1:2000, anti-ATF4 1:2000; anti-XBP-1 1:2000; anti-JNK1 1:5000 (Santa Cruz); anti-caspase-12 1:3000 (Exalpha Biologicals), anti-BAX and anti-BAK 1∶1000 (Upstate Technology); anti-HA 1∶2000 (Roche), anti-caspase-9 1:1000 (Pharmigen), anti-actin 1∶5000 (Chemicon Inc.), anti-SERCA 1∶1000 (Affinity Bioreagents), anti-HSP90 1:1000 (Santa Cruz Biotechnology) and anti-pPERK, and Article Title: RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK–CHOP signaling Article Snippet: Primary antibodies included anti-RCN1 (Bethyl, Montgomery, TX, USA), anti-GFP (MBL, Nagoya, Japan), anti-Flag (Sigma), anti-IP 3 R1 (Bethyl), anti-tubulin (Sigma), anti-ATF4 (GeneTex, San Antonio, TX, USA), anti-pIRE1 (Abcam, Cambridge, MA, USA), anti-pPERK, anti-caspase-3, anti-PERK, anti-IRE1, anti-GAPDH, anti-CHOP, anti-eIF2α and Article Title: Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice Article Snippet: Dilution ratio of primary antibodies used: Article Title: Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling Article Snippet: The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and Article Title: Dual role of sphingosine kinase-1 in promoting the differentiation of dermal fibroblasts and the dissemination of melanoma cells. Article Snippet: Despite progress in the understanding of the biology and genetics of melanoma, no effective treatment against this cancer is available.. The adjacent microenvironment has an important role in melanoma progression.. Defining the molecular signals that control the bidirectional dialog between malignant cells and the surrounding stroma is crucial for efficient targeted therapy. Article Title: Insulin Secretion and Ca 2+ Dynamics in β-Cells Are Regulated by PERK (EIF2AK3) in Concert with Calcineurin Article Snippet: Primary antibodies used in the analysis were: anti-eIF2α-P (1:500, Invitrogen), anti-tubulin (1:1000, Sigma), Article Title: TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways Article Snippet: For anti-BCL-2 (Abcam), anti-BAX (Abcam), and Plasmid Preparation:Article Title: The p53 target DRAM1 modulates calcium homeostasis and ER stress by promoting contact between lysosomes and the ER through STIM1 Article Snippet: Following blocking with 5% nonfat milk at room temperature for 1 h, membranes were incubated with following primary antibodies at 4°C overnight (Anti-DRAM1, Abclonal A15468, 1:1000; Anti-ACTB, Proteintech, 1:3000; Anti-Bax, Santa Cruz sc-526, 1:300; Anti-P53, Santa Cruz sc-6243, 1:300; Anti-Beclin1, Proteintech 11306-1-AP, 1:1000; Anti-CHOP, Proteintech 15204-1-AP, 1:1000; Anti-GRP78, Proteintech 11587-1-AP, 1:1000; Anti-LC3, Proteintech 14600-1-AP, 1:1000; Anti-P62, Proteintech 18420-1-AP, 1:1000; Anti-ATF4, Proteintech 10835-1-AP, 1:1000; Anti-EIF2α, Proteintech 11233-1-AP, 1:1000; Anti-p-EIF2α, Article Title: A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis Pathway Article Snippet: The following antibodies and dilutions were used: anti-CHOP/GADD153 1:2000, anti-ATF4 1:2000; anti-XBP-1 1:2000; anti-JNK1 1:5000 (Santa Cruz); anti-caspase-12 1:3000 (Exalpha Biologicals), anti-BAX and anti-BAK 1∶1000 (Upstate Technology); anti-HA 1∶2000 (Roche), anti-caspase-9 1:1000 (Pharmigen), anti-actin 1∶5000 (Chemicon Inc.), anti-SERCA 1∶1000 (Affinity Bioreagents), anti-HSP90 1:1000 (Santa Cruz Biotechnology) and anti-pPERK, and Article Title: RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK–CHOP signaling Article Snippet: Primary antibodies included anti-RCN1 (Bethyl, Montgomery, TX, USA), anti-GFP (MBL, Nagoya, Japan), anti-Flag (Sigma), anti-IP 3 R1 (Bethyl), anti-tubulin (Sigma), anti-ATF4 (GeneTex, San Antonio, TX, USA), anti-pIRE1 (Abcam, Cambridge, MA, USA), anti-pPERK, anti-caspase-3, anti-PERK, anti-IRE1, anti-GAPDH, anti-CHOP, anti-eIF2α and Article Title: Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice Article Snippet: Dilution ratio of primary antibodies used: Article Title: Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling Article Snippet: The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and Article Title: Dual role of sphingosine kinase-1 in promoting the differentiation of dermal fibroblasts and the dissemination of melanoma cells. Article Snippet: Despite progress in the understanding of the biology and genetics of melanoma, no effective treatment against this cancer is available.. The adjacent microenvironment has an important role in melanoma progression.. Defining the molecular signals that control the bidirectional dialog between malignant cells and the surrounding stroma is crucial for efficient targeted therapy. Article Title: Insulin Secretion and Ca 2+ Dynamics in β-Cells Are Regulated by PERK (EIF2AK3) in Concert with Calcineurin Article Snippet: Primary antibodies used in the analysis were: anti-eIF2α-P (1:500, Invitrogen), anti-tubulin (1:1000, Sigma), Article Title: TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways Article Snippet: For anti-BCL-2 (Abcam), anti-BAX (Abcam), and |
